Figure 5.

Gabapentin and duloxetine inhibited oxaliplatin- and paclitaxel-induced extracellular signal-regulated kinase 1/2 (ERK1/2) activation. Western blotting was conducted to analyze phosphorylated ERK1/2 (phospho-ERK1/2) protein expression in the spinal cord (L4–L6) obtained from mice on day 14 after treatment with oxaliplatin, paclitaxel, gabapentin, or duloxetine. β-actin was used as a protein loading control. Quantification of phospho-ERK1/2 and ERK1/2 expression, normalized against that of β-actin. The results show four independent experiments. ** p < 0.01 vs. controls (Shapiro–Wilk test and ANOVA with Dunnett’s test).