Figure 1.

Encapsulation and 3-D culture of naïve human embryonic stem cells (ESCs) in self-assembling scaffolds. Elf1 cells grown in 2-D culture conditions were dissociated into single cells and mixed with self-assembling end-functionalized polymers, polymers functionalized with thiol (PEG-8-SH) and acrylate (PEG-8-Acr). The polymer/cell mixture was then transferred to a syringe mold and allowed to form 3-D scaffolds, encapsulating the cells. The scaffolds were then transferred to 24-well plates containing culture medium.