Figure 3.

(A) Scheme of the formation of gentamicin and PAA complexes in 500 Å~10⁻³ m NaCl at pH 4.5 (I), LbL assembly of PLL and PAA–gentamicin complexes. The LbL assembly was performed in 500 Å~10⁻³ m NaCl at pH 4.5 in four steps: 1. 15 min incubation of 100 μL drop of 1 mg mL⁻¹ PLL, 2. removal of the PLL that has not been adsorbed by dipping the substrate in 500 Å~10⁻³ m NaCl pH 4.5, 3. 15 min incubation of 100 μL drop of PAA–gentamicin complexes, and 4. removal of the complexes that have not been adsorbed by dipping the substrate in 500 Å~10⁻³ m NaCl pH 4.5. This cycle is repeated four times (II) and scheme of PEMs showing four bilayers of PLL/PAA–gentamicin complexes grown on top of titania films (III), (B) QCM-D monitoring of Layer-by-Layer assembly of PLL and PAA–gentamicin complexes and (C) I. S. aureus growth on top of the multilayer and II. on glass substrates immersed in gentamicin. Cell observer images following 24 h of incubation of S. aureus on I. top of the multilayer and II. glass immersed in gentamicin, insert images are a zoom of 4×, and III. CFU of the adhered bacteria on the PEM and glass with gentamicin. Reproduced with permission from [101], John Wiley and Sons, Inc, 2019.