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. 2020 Dec 24;13(1):16. doi: 10.3390/pharmaceutics13010016

Figure 6.

Figure 6

Strategies for assembling antiadhesive multilayer films. (A) AFM images of nonannealed and annealed (CHI/HA)7CHI multilayers. (B) AFM line profile from images depicted in (A) as indicated by the dashed line. (C) Colony-forming units (CFU) for S. aureus and E. coli bacteria detached from glass, nonannealed and annealed (CHI/HA)7CHI PEMs. (D) C2C12 cell images 24 h post seeding on nonannealed and annealed (CHI/HA)7CHI PEMs. (E) Live staining of adherent S. aureus on non-crosslinked LbL films with varying surface isoelectric point (pI) and water contact angle (θ). Scale bar is 100 µm. (F) Fluorescence microscopy images of adherent fibroblast cells stained with DAPI and phalloidin after 2 day seeding on non-crosslinked LbL films with varying surface isoelectric point (pI) and water contact angle (θ). Scale bar is 100 µm. (A,B) adapted with permission from [131], American Vacuum Society, 2017. (C,D) adapted with permission from [130], Elsevier, 2017. (E,F) adapted with permission from [132], American Chemical Society, 2018.