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Optimization of tandem mass spectrometry (MS/MS) ion transitions and other parameters for detection of the target antibiotics.
| Compound. | Ciprofloxacin | Levofloxacin |
|---|---|---|
| Retention time (min) | 6.72 | 6.49 |
| Precursor ion [M + H]+ | 332 | 362 |
| Source potential (V) | 50 | 50 |
| Collision Energy (eV) | 20 | 20 |
| MRM1 transition | 332 > 288 | 362 > 318 |
| MRM2 transition | 332 > 314 | 362 > 261 |
