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. 2020 Dec 30;13(1):101. doi: 10.3390/nu13010101

Table 1.

Breast milk fatty acids concentrations according to the stage of lactation (T1, T2 & T3).

Stage of Lactation
Breast Milk Fatty Acids Contents
% of Total Fatty Acids
Transitional Milk (T1)
2–3 Weeks
(n = 8)
Early Milk (T2)
>3–8 Weeks
(n = 26)
Mature Milk (T3)
>8–16 Weeks
(n = 26)
Mean ± SD
6:0 (Caproic acid) 0.11 ± 0.02 0.12 ± 0.01 0.13 ± 0.01
8:0 (Caprylic acid) 0.42 ± 0.03 0.45 ± 0.03 0.42 ± 0.02
10:0 (Capric acid) 2.29 ± 0.23 2.13 ± 0.15 2.09 ± 0.07
12:0 (Lauric acid) 9.61 ± 0.84 a 8.14 ± 0.77 b 8.96 ± 0.46 a,b
14:0 (Myristic acid) 7.22 ± 0.55 a 6.20 ± 0.55 b 7.42 ± 0.41 a
14:1 n-5 (Myristoleic acid) 0.04 ± 0.01 a 0.13 ± 0.01 b 0.09 ± 0.01 a,b
16:0 (Palmitic acid) 25.2 ± 1.25 a 26.3 ± 0.35 b 26.8 ± 0.47 b
16:1 n-7 (Palmitoleic acid) 3.09 ± 0.38 3.26 ± 0.19 2.61 ± 0.17
18:0 (Stearic acid) 4.26 ± 0.04 4.81 ± 0.14 4.70 ± 0.13
18:1 n-7 (Vaccenic acid) 1.58 ± 0.26 1.85 ± 0.06 1.53 ± 0.09
18:1 n-9 (Oleic acid) 33.0 ± 1.11 a,b 33.7 ± 0.81 a 32.5 ± 0.47 b
18:2 n-6 (Linoleic acid) 10.1 ± 0.60 10.1 ± 0.32 9.83 ± 0.26
18:3 n-3 (α-Linolenic acid) 0.44 ± 0.2 0.35 ± 0.02 0.32 ± 0.03
18:3 n-6 (γ-Linolenic acid) 0.09 ± 0.02 0.09 ± 0.01 0.08 ± 0.01
20:0 (Arachidic acid) 0.14 ± 0.01 0.15 ± 0.01 0.16 ± 0.01
20:1 n-9 (Eicosenoic acid) 0.31 ± 0.04 0.30 ± 0.01 0.29 ± 0.02
20:2 n-6 (Eicosadienoic acid) 0.20 ± 0.01 0.20 ± 0.01 0.19 ± 0.02
20:3 n-6 (Dihomo--linolenic acid) 0.33 ± 0.04 0.31 ± 0.02 0.30 ± 0.02
20:4 n-6 (Arachidonic acid) (ARA) 0.43 ± 0.04 0.38 ± 0.02 0.37 ± 0.02
20:5 n-3 (Eicosapentaenoic acid) (EPA) 0.05 ± 0.01 0.06 ± 0.01 0.07 ± 0.01
22:0 (Behenic acid) 0.07 ± 0.01 0.07 ± 0.01 0.08 ± 0.01
22:4 n-6 (Docosatetraenoic acid) 0.10 ± 0.01 0.08 ± 0.01 0.08 ± 0.01
22:5 n-3 (Docosapentaenoic acid) 0.11 ± 0.01 0.11 ± 0.01 0.13 ± 0.01
22:5 n-6 (Docosapentaenoic acid) 0.01 ± 0.01 0.06 ± 0.01 0.07 ± 0.01
22:6 n-3 (Docosahexaenoic acid) (DHA) 0.45 ± 0.06 0.47 ± 0.05 0.56 ± 0.05
24:0 (Lignoceric acid) 0.07 ± 0.01 0.07 ± 0.01 0.08 ± 0.01
24:1 n-9 (Nervonic acid) 0.07 ± 0.01 0.07 ± 0.01 0.07 ± 0.01
Sum SFA 49.4 ± 1.55 a 48.5 ± 1.20 a 50.8 ± 0.78 b
Sum MUFA 38.2 ± 1.13 a 39.3 ± 0.95 a 37.1 ± 0.61 b
Sum PUFA 12.4 ± 0.79 12.2 ± 0.32 12.0 ± 0.34
Sum n-3 1.06 ± 0.16 0.99 ± 0.06 1.09 ± 0.08
Sum n-5 0.04 ± 0.01 a 0.13 ± 0.01 b 0.09 ± 0.01 a,b
Sum n-6 11.3 ± 0.65 11.2 ± 0.33 10.9 ± 0.29
Sum n-7 4.71 ± 0.47 a,b 5.11 ± 0.24 a 4.16 ± 0.24 b
Sum n-9 33.5 ± 1.14 a,b 34.1 ± 0.82 a 32.9 ± 0.48 b
n-6 to n-3 ratio 10.7 ± 3.92 11.3 ± 5.79 10.1 ± 3.77
ARA to DHA ratio 0.96 ± 0.66 a 0.80 ± 0.34 a 0.66 ± 0.41 b

Mean values in the same row with different alphabets indicate significant difference (p < 0.05) based on ANOVA and post hoc LSD tests. Saturated Fatty Acids (SFA): 6:0 (Caproic acid), 8:0 (Caprylic acid), 10:0 (Capric acid), 12:0 (Lauric acid), 14:0 (Myristic acid), 16:0 (Palmitic acid), 18:0 (Stearic acid), 20:0 (Arachidic acid), 22:0 (Behenic acid), 24:0 (Lignoceric acid). Monounsaturated Fatty Acids (MUFA): 14:1 n-5 (Myristoleic acid), 16:1 n-7 (Palmitoleic acid), 18:1 n-7 (Vaccenic acid), 18:1 n-9 (Oleic acid), 20:1 n-9 (Eicosenoic acid), 24:1 n-9 (Nervonic acid). Polyunsaturated Fatty Acids (PUFA): 18:2 n-6 (Linoleic acid), 18:3 n-3 (α-Linolenic acid), 18:3 n-6 (γ-Linolenic acid), 20:2 n-6 (Eicosadienoic acid), 20:3 n-6 (Dihomo-linolenic acid), 20:4 n-6 (Arachidonic acid), 20:5 n-3 (Eicosapentaenoic acid), 22:4 n-6 (Docosatetraenoic acid), 22:5 n-3 (Docosapentaenoic acid), 22:5 n-6 (Docosapentaenoic acid), 22:6 n-3 (Docosahexaenoic acid).