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(A) Feces spiked with PepC. Singly charged ion at m/z 2912.3 [M + H]+ and the doubly charged one at m/z 1457.1 [M + 2H]2+ (B) Spectra from the reaction of feces spiked with BoNT/C at a concentration of 16 mLD50/500 µL and PepC (15 mM). Intact PepC at m/z 2912.3 [M + H]+ with the doubly charged ion [M + 2H]2+at m/z 1457.1, cleavage products are not clearly visible.
