Table 3.

Comparison of the MBA and EndoPep-MS using spiked samples.

Concentration		EndoPep-MS			MBA
(mLD50/500 µL)		Replicate 1	Replicate 2	Replicate 3	Mouse 1	Mouse 2
			BoNT/C
Serum	4	Positive	Positive	Positive	Expired	Expired
	2	Positive	Positive	Positive	Expired	Expired
	1	Positive	Positive	Positive	Expired	Expired
	0.5	Negative	Negative	Negative	Expired	Expired
	0.25	Negative	Negative	Negative	Survived	Survived
	Negative	Negative	Negative	Negative	NT	NT
Broth culture	4	Positive	Positive	Positive	Expired	Expired
	2	Positive	Positive	Positive	Expired	Survived
	1	Negative	Negative	Negative	Survived	Survived
	0.5	Negative	Negative	Negative	Survived	Survived
	0.25	Negative	Negative	Negative	Survived	Survived
	Negative	Negative	Negative	Negative	NT	NT
Feces (1:1 PGB)	4	N/A	N/A	N/A	Expired	Expired
	2	N/A	N/A	N/A	Survived	Survived
	1	N/A	N/A	N/A	Survived	Survived
	0.5	N/A	N/A	N/A	Survived	Survived
	0.25	N/A	N/A	N/A	Survived	Survived
	Negative	N/A	N/A	N/A	NT	NT
BoNT/D
Serum	4	Positive	Positive	Positive	Expired	Expired
	2	Positive	Positive	Positive	Expired	Expired
	1	Positive	Positive	Positive	Survived	Survived
	0.5	Positive	Positive	Negative	Survived	Survived
	0.25	Negative	Negative	Negative	Survived	Survived
	Negative	Negative	Negative	Negative	NT	NT
Broth culture	4	Positive	Positive	Positive	Expired	Expired
	2	Positive	Positive	Positive	Expired	Expired
	1	Negative	Negative	Negative	Expired	Expired
	0.5	Negative	Negative	Negative	Survived	Survived
	0.25	Negative	Negative	Negative	Survived	Survived
	Negative	Negative	Negative	Negative	NT	NT
Feces (1:1 PGB)	4	Positive	Positive	Positive	Expired	Expired
	2	Positive	Positive	Positive	Expired	Survived
	1	Negative	Negative	Negative	Survived	Survived
	0.5	Negative	Negative	Negative	Survived	Survived
	0.25	Negative	Negative	Negative	Survived	Survived
	Negative	Negative	Negative	Negative	NT	NT

N/A: not applicable; NT: not tested.