Table 4.
Comparison between the MBA, EndoPep-MS method, and qPCR applied in positive and negative diagnostic samples.
| Sample ID | Origin | BoNTs | EndoPep-MS | ||
|---|---|---|---|---|---|
| MBA | qPCR | BoNT/C | BoNT/D | ||
| GPB extracts | |||||
| 8/4/15 | Vegetables + meat | NEG | / | NEG | NEG |
| 8/3/15 | Vegetables + meat | NEG | / | NEG | NEG |
| 8/6/14 | Vegetables + meat | BoNT/E | / | NEG | NEG |
| 8/7/14 | Vegetables + meat | BoNT/A | / | NEG | NEG |
| 8/8/15 | Vegetables + meat | BoNT/A | / | NEG | NEG |
| 8/10/15 | Vegetables + meat | BoNT/A | / | NEG | NEG |
| 8/9/15 | Vegetables + meat | NEG | / | NEG | NEG |
| 8/1/15 | Vegetables + meat | NEG | / | NEG | NEG |
| 3/1/15 | Vegetables + meat | BoNT/B | / | NEG | NEG |
| 8/2/15 | Vegetables + meat | BoNT/A | / | NEG | NEG |
| Enrichment cultures | |||||
| 45/2/12 | Mushrooms in oil | NEG | / | NEG | NEG |
| 3/1/15 | Eggplants in oil | BoNT/B | B | NEG | NEG |
| 43/1/12 | Human feces | NEG | / | NEG | NEG |
| 19/10/11 | Rectal swab | BoNT/E | E | NEG | NEG |
| 1/4/15 | Mushrooms in oil | BoNT/B | B | NEG | NEG |
| 2/2/15 | Human feces | NEG | / | NEG | NEG |
| 8/8/08 | Human feces | BoNT/B | B | NEG | NEG |
| 48/4/12 | Human feces | NEG | / | NEG | NEG |
| 18/5/12 | Olives | BoNT/B | B | NEG | NEG |
| 18/5/12 | Sausages | NEG | / | NEG | NEG |
| 21/19/12 | Tuna in oil | NEG | / | NEG | NEG |
| 55/6/11 | Eggplants in oil | NEG | / | NEG | NEG |
| 153/113 | Truffle cream | NEG | / | NEG | NEG |
| 20/3/12 | Chili pepper in oil | NEG | / | NEG | NEG |
| 16/3/12 | Human feces | BoNT/B | B | NEG | NEG |
| 166/2/13 | Human feces | NEG | / | NEG | NEG |
| 1556/15 | Tuna | BoNT/B | B | NEG | NEG |
| 16/4/12 | Mushrooms in oil | BoNT/B | B | NEG | NEG |
| 22/2/14 | Human feces | BoNT/B | B | NEG | NEG |
| 22/4/14 | Human feces | BoNT/B | B | NEG | NEG |
| 46/3/14 | Human feces | BoNT/B | B | NEG | NEG |
| 152/1/10 | Truffle cream | BoNT/B | B | NEG | NEG |
| 47/2/15 | Human feces | BoNT/B | B | NEG | NEG |
| 11/3/15 | Mushrooms in oil | BoNT/B | B | NEG | NEG |
| 18/2/15 | Human feces | BoNT/B | B | NEG | NEG |
| 42/2/15 | Human feces | BoNT/B | B | NEG | NEG |
| 4871/15 | Human feces | NEG | / | NEG | NEG |
| 49/2/15 | Human feces | BoNT/B | B | NEG | NEG |
| 5271715 | Chili pepper + tuna in oil | NEG | / | NEG | NEG |
| 5509/1/20 | Wood shavings | BoNT/C + BoNT/D | C + D | POS | POS |
| 5509/2/20 | Litter | BoNT/C + BoNT/D | C + D | POS | POS |
| 4888/20 | Quail | BoNT/D | D/C | NEG | POS |
| 5291/1/20 | Water (drinking) | BoNT/D | D/C | NEG | POS |
| 5291/2/20 | Water (tank) | BoNT/D | D/C | NEG | POS |
| 5729/1/20 | Bovine | BoNT/D | D | NEG | POS |
| 5729/2/20 | Bovine | BoNT/D | D | NEG | POS |
| 6126/2/20 | Water | BoNT/D | D | NEG | POS |
| 6126/3/20 | Water | BoNT/D | D | NEG | POS |
| 6126/7/20 | Quail | BoNT/D | D | NEG | POS |
| 6126/8/20 | Quail | BoNT/D | D | NEG | POS |
| 6126/10/20 | Quail | BoNT/D | D | NEG | POS |
| 6126/11/20 | Quail | BoNT/D | D | NEG | POS |
| Sera | |||||
| 5674/10 | Duck | BoNT/C | / | POS | NEG |
| 8103/2/09 | Broiler | BoNT/C | / | POS | NEG |
| 8103/10/09 | Broiler | NEG | / | NEG | NEG |
| 8103/13/09 | Broiler | BoNT/C | / | POS | NEG |
| 2659/2/10 | Broiler | NEG | / | NEG | NEG |
| 2659/4/10 | Broiler | NEG | / | POSw | NEG |
| 2659/5/10 | Broiler | BoNT/C | / | POS | NEG |
| 4691/11 | Turkey | BoNT/C | / | POS | NEG |
| 4863/13 | Pheasant | BoNT/C | / | POS | NEG |
| 474/14 | Dog | BoNT/C | / | POS | NEG |
| 5313/28/14 | Broiler | BoNT/C | / | POS | NEG |
| 5313/29/14 | Broiler | BoNT/C | / | POS | NEG |
| 5313/30/14 | Broiler | BoNT/C | / | POS | NEG |
| 6289/3/14 | Mallard | NEG | / | NEG | NEG |
| 6289/4/14 | Mallard | NEG | / | NEG | NEG |
| 5993/15 | Pheasant | BoNT/C | / | POS | NEG |
| 6353/15 | Broiler | BoNT/C | / | POS | NEG |
| 6660/1/15 | Broiler | BoNT/C | / | POS | NEG |
| 6660/2/15 | Broiler | BoNT/C | / | POS | NEG |
| 6841/2/15 | Bovine | NEG | / | NEG | NEG |
| 6841/3/13 | Bovine | NEG | / | POSw | NEG |
| Feces | |||||
| 5641/12 | Swan | BoNT/C | / | POS | NEG |
| Milk | |||||
| 9861/51/18 | Bovine | NEG | / | NEG | NEG |
| 9861/189/18 | Bovine | NEG | / | NEG | NEG |
| 9861/242/18 | Bovine | NEG | / | NEG | NEG |
| 9861/251/18 | Bovine | NEG | / | NEG | NEG |
| 9861/258/18 | Bovine | NEG | / | NEG | NEG |
w = weak; POS: positive; NEG: negative.