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. 2020 Dec 28;9(1):60. doi: 10.3390/microorganisms9010060

Figure 3.

Figure 3

(A) Analysis of the anti-biofilm effect of eicosanoic acid (C20:0) alone, with Xccφ1, and in combination with Xccφ1 and HA on Xcc mature biofilm. CLSM analysis was performed on 72 h mature Xcc biofilms after 3 h of treatment at Room Temperature. The biofilm structures were stained using the LIVE/DEAD® Biofilm Viability Kit. (B) Evaluation of biofilm formation of Xcc in dynamic conditions in the presence and absence of C20:0 and the complex of HA-Xccφ1+C20:0. Biofilm formation was performed in a three-channel flow cell for 48 h. CLSM analysis was performed after 3 h incubation at RT in the presence and the absence of the anti-biofilm samples.