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. 2021 Jan 4;9(1):35. doi: 10.3390/biomedicines9010035

Figure 2.

Figure 2

Figure 2

Rhosin inhibits RhoA/C-YAP pathway in B16BL6 and 4T1 cells: (a) B16BL6 and 4T1 cells were treated with rhosin at indicated concentration for 3 days. Images of Western blots for the RhoA pull-down, RhoA, RhoC pull-down, RhoC, phospho-ERK1/2, ERK1/2, phospho-Akt, Akt, phospho-NF-κB, NF-κB, YAP, β-actin, and lamin A/C; (b) Quantification of the amounts of RhoA pull-down, RhoC pull-down, phospho-ERK1/2, phospho-Akt, phospho-NF-κB, and YAP after normalization to the amounts of corresponding protein. The results are representative of 4 independent experiments. * p < 0.01 vs. controls (ANOVA with Dunnett’s test); (c,d) B16BL6 cells were treated with negative siRNA, (c) RhoA siRNA, or (d) RhoC siRNA. Images of Western blots for the RhoA, RhoC, YAP, β-actin, and lamin A/C. Quantification of the amounts of RhoA, RhoC, and YAP after normalization to the amounts of corresponding protein. The results are representative of 4 independent experiments. * p < 0.01 vs. controls (ANOVA with Dunnett’s test).