Figure 3.
Type I and III interferons (IFNs) protect against Coxsackievirus B3 (CVB3) infection in intestinal epithelial cells (IECs). (a) CaCo-2 and (b) HT-29 cells were treated with vehicle, IFNλ1, IFNλ2 (both at concentration of 100 ng/mL), or IFNα (1000 U/mL) for 24 h and were then infected with CVB3 at a multiplicity of infection (MOI) of 10−4 for 24 h. Virus titers in cells and supernatants were measured 24 h p.i. by standard plaque assay. Data are presented as the mean plaque forming units (PFUs) ± SD from at least four independent experiments per cell line. Significant decreases in virus titers were observed in all treatments when compared to the vehicle alone (*** p < 0.001; one-way ANOVA).