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. 2021 Jan 5;9(1):105. doi: 10.3390/microorganisms9010105

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Polyinosinic:polycytidylic acid (poly (I:C)) transfection, but not Coxsackievirus B3 (CVB3) infection, induces the expression of interferons (IFNs) in intestinal epithelial cells (IECs). (a) The expression of the pattern recognition receptors toll-like receptor 3 (TLR3), melanoma differentiation-associated protein 5 (MDA-5), and retinoic acid-inducible gene 1 (RIG-I) were confirmed in CaCo-2 and HT-29 cells at the mRNA level by real-time PCR. (b) CaCo-2 or (c) HT-29 cells were treated with 10 ng/mL or 30 ng/mL poly (I:C) (i) or transfected with 10 ng/mL poly (I:C) (ii) or infected with 0.01 or 1 plaque forming units (PFU)/mL CVB3 (iii) for 3 h or 6 h. The expression of the type I or III IFNs at the mRNA level was measured by real-time PCR. Data presented as the mean ± SD from at least three independent experiments per cell line (* p < 0.05 and ** p < 0.01; Student’s t-test with Welch’s correction or one-way ANOVA).

Polyinosinic:polycytidylic acid (poly (I:C)) transfection, but not Coxsackievirus B3 (CVB3) infection, induces the expression of interferons (IFNs) in intestinal epithelial cells (IECs). (a) The expression of the pattern recognition receptors toll-like receptor 3 (TLR3), melanoma differentiation-associated protein 5 (MDA-5), and retinoic acid-inducible gene 1 (RIG-I) were confirmed in CaCo-2 and HT-29 cells at the mRNA level by real-time PCR. (b) CaCo-2 or (c) HT-29 cells were treated with 10 ng/mL or 30 ng/mL poly (I:C) (i) or transfected with 10 ng/mL poly (I:C) (ii) or infected with 0.01 or 1 plaque forming units (PFU)/mL CVB3 (iii) for 3 h or 6 h. The expression of the type I or III IFNs at the mRNA level was measured by real-time PCR. Data presented as the mean ± SD from at least three independent experiments per cell line (* p < 0.05 and ** p < 0.01; Student’s t-test with Welch’s correction or one-way ANOVA).