Figure 4.

The application of blue LED light does not modify ramp-evoked currents in both cultured fibroblasts and HaCat cells: (A) original whole-cell patch clamp current traces evoked by a voltage ramp protocol (from $-80$ to $+80$ mV, 800 ms, top left inset) before (Ctrl, black trace) or 5 min after the application of 20.6 J/cm${}^2$ blue LED light (BLL, grey trace) in a typical fibroblast (HF); (B) averaged time courses (mean ± SEM) of ramp-evoked currents at $+80$ mV, expressed as percent of ctrl, in HFs (n = 7) before, during or after the application of blue LED light; (C) pooled data (mean ± SEM) of ramp current amplitude at $+80$ mV, recorded before or after 5 min of blue LED light (BLL) application in HFs (n = 7); (D) original whole-cell patch clamp current traces evoked by a voltage ramp protocol (from $-80$ to $+120$ mV, 800 ms, top left inset) before (Ctrl, black trace) or 5 min after the application of 20.6 J/cm${}^2$ blue LED light (BLL, grey trace) in a typical HaCat cell; (E) averaged time courses (mean ± SEM) of ramp-evoked currents at $+120$ mV, expressed as percent of ctrl, in HaCat cells (n = 6) before, during or after the application of 20.6 J/cm${}^2$ fluence of blue LED light; and (F) pooled data (mean ± SEM) of ramp current amplitude at $+120$ mV, recorded before or after 5 min of blue LED light (BLL) application in HaCat (n = 6).