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. 2021 Jan 22;16:3. doi: 10.1186/s13024-021-00423-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Cryopreservation of human cerebrospinal fluid cells retains cellular viability. a Workflow for cryopreservation of CSF cells for scRNA-TCRseq. b Gating strategy for sorting live cryopreserved cells by flow cytometry. c Quantification of live cell count (live singlets) from flow cytometry sorting following cell thawing. Mean ± s.e.m. d Quantification of viability (percentage of live singlets out of all singlets) from sorting by flow cytometry. Mean ± s.e.m. e Linear regression of live cell count post thaw versus length of storage. f Quantification of the number of CSF cells captured for sequencing