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. 2021 Jan 5;10(1):93. doi: 10.3390/plants10010093

Figure 1.

Figure 1

Delivery of dsRNA to citrus phloem through laser micro-perforation. (a–c) Fluorescence micrographs of laser-perforated areas on citrus leaves and applied with H2O, deoxyglucose, 2-NBDG, and CY-3 labeled dsRNA-PDS, respectively. (d–f) cross sections of laser-treated citrus leaf petioles and applied with H2O, deoxyglucose, 2-NBDG, and CY-3 labeled dsRNA-PDS, respectively. (g–i) cross sections of lased-treated citrus blades and applied with H2O, deoxyglucose, 2-NBDG, and CY-3 labeled dsRNA-PDS, respectively. Note the localization of fluorescent deoxyglucose, 2-NBDG, and the CY-3 labeled dsRNA molecules are localized in the phloem (e,f). Control leaves were laser-treated, then water was applied instead of dsRNA or fluorescent glucose.