Figure 2.

Variation in the abundance of the candidate reference gene transcripts over all life stages of Megachile rotundata expressed as Cq values for (A) genes with low variation in expression levels, (B) genes with intermediate variation in expression level, and (C) genes with the greatest variation in expression. Life stages are 1= Egg, 2 = 2nd instar, 3 = 3rd instar, 4 = 4th instar, 5 = 5th instar (prior to cocoon formation), 6 = non-diapausing prepupa, 7 = diapausing prepupa, 8 = post-diapause pupa, 9 = non-diapause pupa, 10 = Adult female, and 11 = Adult male.