. 2021 Jan 6;12(1):36. doi: 10.3390/insects12010036

Table 1.

Detailed information regarding the primers of 12 candidate genes.

Gene	Genbank Accession Number	Primer Sequences Forward and Reverse (5′ to 3′)	Amplicon Length	T_m (°C)	% GC	Coding Protein
ACT	SRX040740	CGGTTCGTGATAGGATTCATTGGTGGT	64	60.4	48.1	ß-actin
	SRX040740	TGGGAAGACATGTGTCATGTATGGGA	64	59.6	46.1	ß-actin
APN	GD241616	TGCGCTGGACTGAGGAACGC	72	62.8	65.0	Aminopeptidase N
	GD241616	GGCAAGCCAAGAGCGCGAAC	72	62.3	65.0	Aminopeptidase N
Cr-PII	GD241657	TGGTCCGCAAGGTCGAAGCC	103	62.7	65.0	Cr-PII allergens
	GD241657	GCGGAAGCCGGTTGGTGGAA	103	63.3	65.0	Cr-PII allergens
GAPDH	GD240938	GTGCCGCCAAAGCTGTCGGT	81	63.6	65.0	Glyceraldehyde-3-phosphate dehydrogenase
	GD240938	GCTGGTTTGCCAAGTCTGACCGT	81	62.1	56.5	Glyceraldehyde-3-phosphate dehydrogenase
GRX	GD240911	ATTGGGCGACATGACCGGCG	76	63.2	65.0	Glutaredoxin (GRX) family
	GD240911	AGCTTCTTCTGCAGCTCGCCA	76	61.6	57.1	Glutaredoxin (GRX) family
NPC2	GD241023	CGCACTCGTCCTGGTAGCGT	108	61.9	65.0	Niemann-pick type C2 (Npc2)
	GD241023	CGTGCTTCGACATCGGTTCCCC	108	60.7	61.9	Niemann-pick type C2 (Npc2)
PMIIM	GD241063	ACGCGACCGTCCATGTCTGTG	66	62.1	61.9	Peritrophic matrix insect intestinal mucin
	GD241063	GTAAGCCGGTCCATCGGCCA	66	62.3	65.0	Peritrophic matrix insect intestinal mucin
RPL8	GD240977	GGAGCATCCTCACGGTGGTGG	71	62.5	66.6	Ribosomal protein L8
	GD240977	TCCGGTACGACGAGCAGCAA	71	61.1	60.0	Ribosomal protein L8
RPL27A	GD240974	GGGGACTGCGATCAAGATGTCAAC	93	59.8	54.1	Ribosomal protein L27A
	GD240974	GGTGGTGCAACCCACCAGCA	93	63.4	65.0	Ribosomal protein L27A
RPS5	GD240987	CGGTCGCGCTGGTACTGTCA	64	62.1	65.0	Ribosomal protein S5
	GD240987	ATGCGGCTTCCCTAGCACCG	64	62.6	65.0	Ribosomal protein S5
RPS18	GD240984	ACGAATTGGCAAGATGTCGCTCGT	91	61.2	50.0	Ribosomal protein S18
	GD240984	GCATAACGGCGACCAACACC	91	59.3	60.0	Ribosomal protein S18
TarRNA	GD241002	GCGGTTAACGCCGCTGGGAA	80	63.3	65.0	Transcript antisense to ribosomal RNA
	GD241002	CTACGGGCCTGGCACCCTCT	80	64.1	70.0	Transcript antisense to ribosomal RNA