Table 2.
Comparison of stability of transcript abundance by NormFinder and BestKeeper among larval, prepupal, and adult stages of Megachile rotundata.
| Larval Stage | Prepupal Stage | Adult Stages | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene Name |
Mean Rank |
BestKeeper | NormFinder | BestKeeper | NormFinder | BestKeeper | NormFinder | ||||||
| SD 1 | Rank | SV 2 | Rank | SD | Rank | SV | Rank | SD | Rank | SV | Rank | ||
| RPS18 | 1 | 1.12 | 4 | 0.03 | 2 | 0.54 | 4 | 0.05 | 2 | 0.94 | 4 | 0.03 | 5 |
| RPS5 | 2 | 1.04 | 3 | 0.05 | 5 | 0.52 | 3 | 0.07 | 10 | 0.83 | 2 | 0.02 | 1 |
| RPL27A | 3 | 1.35 | 5 | 0.02 | 1 | 0.44 | 2 | 0.06 | 5 | 1.06 | 6 | 0.04 | 6 |
| GRX | 4 | 1.80 | 11 | 0.04 | 4 | 0.74 | 7 | 0.02 | 1 | 0.92 | 3 | 0.03 | 3 |
| RPL8 | 5 | 0.98 | 1 | 0.05 | 6 | 0.62 | 5 | 0.06 | 7 | 1.14 | 8 | 0.03 | 4 |
| TarRNA | 6 | 1.01 | 2 | 0.10 | 12 | 0.20 | 1 | 0.05 | 3 | 1.00 | 5 | 0.09 | 10 |
| GAPDH | 7 | 1.39 | 6 | 0.04 | 3 | 0.66 | 6 | 0.07 | 9 | 1.09 | 7 | 0.04 | 7 |
| PMIIM | 8 | 1.75 | 10 | 0.07 | 9 | 2.36 | 9 | 0.06 | 6 | 0.56 | 1 | 0.06 | 9 |
| ACT | 9 | 1.54 | 9 | 0.07 | 8 | 1.19 | 8 | 0.06 | 4 | 1.24 | 9 | 0.05 | 8 |
| APN | 10 | 1.41 | 7 | 0.06 | 7 | 3.53 | 12 | 0.12 | 12 | 1.25 | 10 | 0.03 | 2 |
| NPC2 | 11 | 1.42 | 8 | 0.09 | 11 | 2.48 | 10 | 0.07 | 8 | 2.95 | 11 | 0.11 | 11 |
| Cr-PII | 12 | 2.30 | 12 | 0.08 | 10 | 3.36 | 11 | 0.11 | 11 | 3.66 | 12 | 0.15 | 12 |
1 SD (standard deviations): the ideal standard deviation should be less than 1 (Pfaffl et al., 2004); 2 SV (stability values): A low stability value indicates highly stable abundance of transcription for the gene (Pfaffl et al., 2004).