Table 3.
Statistical analysis for stability of transcript abundance among different life stages of Megachile rotundata.
| Average | BestKeeper | NormFinder | |||
|---|---|---|---|---|---|
| Gene | Rank | Rank | SD 1 | Rank | SV 2 |
| RPS18 | 1 | 4 | 1.22 | 2 | 0.06 |
| RPL8 | 2 | 2 | 1.14 | 5 | 0.07 |
| GAPDH | 3 | 7 | 1.74 | 1 | 0.05 |
| RPS5 | 4 | 3 | 1.17 | 6 | 0.07 |
| GRX | 4 | 6 | 1.55 | 3 | 0.06 |
| RPL27A | 4 | 5 | 1.48 | 4 | 0.06 |
| TarRNA | 5 | 1 | 1.00 | 10 | 0.14 |
| ACT | 6 | 8 | 1.53 | 8 | 0.08 |
| APN | 7 | 9 | 2.79 | 7 | 0.07 |
| PMIIM | 8 | 10 | 4.26 | 9 | 0.12 |
| NPC2 | 9 | 11 | 4.54 | 11 | 0.14 |
| Cr-PII | 10 | 12 | 5.00 | 12 | 0.18 |
1 SD (standard deviations): the ideal standard deviation should be less than or close to 1 (Pfaffl et al., 2004); 2 SV (stability values): A low stability value indicates highly stable levels of transcription across the life stages (Pfaffl et al., 2004).