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Percentage of observed mortality at 120 hpf for embryonic zebrafish exposed to particle suspensions with their (a) chorion removed and (b) with chorion intact; concentration-response curves showing predicted mortality at 120 hpf for the groups exposed to Ch-LNPs with (c) chorion removed and (d) chorion intact; a sigmoidal relationship was fit to experimental data to estimate LC50 values and 95 percent confidence intervals.
