Figure 3.

Untranslated region (UTR)-mutation analysis of SLC35F2 by luciferase reporter assay. (A) Schematic view of the whole UTR sequencing cloned in luciferase reporter. The wildtype and mutated 5′UTR sequences were cloned upstream, while the wildtype and mutated 3′UTR were cloned downstream of the NLucP reporter. The bladder cancer cell lines T24 (n = 4) and TCCsup (n = 4) were transfected with the luciferase plasmids and after 24 h luciferase activity (B) and mRNA (C) were analysed. The Luc2 luciferase was used for normalisation to eliminate variations from transfection. The data are expressed as mean ± standard deviation and statistical analysis was performed by non-parametric Mann–Whitney U-test with * p-value < 0.05. Values obtained from wild type sequences were set arbitrarily as 1. PGK: phosphoglycerate kinase, MCS: Multiple Cloning Site.