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. 2021 Jan 22;9:23. doi: 10.1186/s40168-020-00975-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — YM utilization by Bt^Bov isolates. Images of a 100 μm on food particles and b 10 μm filtered rumen extract incubated with FLA-YM and stained with DAPI and Bacteroides-FISH probe (FISH-CF968). Cells were visualized by epifluorescence microscopy. c Counts of cells from FLA-YM incubated rumen extract stained with DAPI, FISH-CF968, FLA-YM, and both FISH-CF968 and FLA-YM. Mean ± standard deviation shown. d 16S rRNA metagenomics sequencing data of extracted rumen communities showing most prevalent phyla (left) and the distribution of Bacteroidetes spp. (right). N = 4