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. 2021 Jan 22;9:23. doi: 10.1186/s40168-020-00975-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — RNA-seq analysis of BtVPI-5482, MD33_MG, and MD40_HG cultured in YM. a Log₂ expression ratios of each transcript of BtVPI-5482, MD33_MG, and MD40_HG. Length of heap map is representative of genome size (BtVPI-5482 = 6.26 Mb, MD33_MG = 6.28 Mb, MD40_HG = 6.16 Mb). Location of MAN-PULs and MD40-PUL55 (white star) shown. Yellow indicates an increase in transcript expression compared to the control, while blue is decreased expression. b TPM values of susC-like genes from MAN-PUL1/2/3 showing if values within each gene are significantly different between the strains; different letters represent statistically significant values (e.g., A vs B) for each separate gene histogram; asterisk indicates SPII predicted proteins. Log₂ fold change compares gene expression of YM cultures normalized to mannose, N = 3, p ≤ 0.01; except BT_3853 and HMNG-PUL, gene expression is not significantly (p > 0.05) different between the two treatments. Significantly different (p < 0.05) TPM expression of the susC-like genes between BtVPI-5482 and Bt^Bov strains; statistical comparison of other MAN-PUL genes between strains were not calculated