Fig. 5.

Induction of chemokine and cytokine expression by EGFR/ERBB and MEK inhibitor treatment in B4B8 cells. a B4B8 cells were treated with DMSO, AZD8931 (100 nM) or trametinib (10 nM) for 1–9 days. Conditioned media was collected and submitted to ELISA for CXCL10, IL6 and TGFB2. The data are the means and SEM three independent experiments (CXCL10) or single determinations at 6 distinct time points (IL6 and TGFB2) and presented as pg per µg of cellular protein. b B4B8 cells were treated for the indicated time with DMSO or AZD8931 (100 nM) and conditioned media was submitted to Luminex analysis for the indicated secreted analytes. The data points represent single determinations at four distinct time points per treatment