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. Author manuscript; available in PMC: 2021 Jan 23.
Published in final edited form as: Methods Mol Biol. 2019;1966:107–124. doi: 10.1007/978-1-4939-9195-2_9

Figure 5.

Figure 5.

Fluorescently-labeled AF1521tandem can detect ADP-ribosylated AR by blot overlay. A) Extracts from androgen (R1881)- or vehicle-treated PC3-AR cells were analyzed by blotting using anti-AR, anti-tubulin, and fluorescently-labeled AF1521tandem probe (Fl-AF1521). As indicated, Fl-AF1521 was pre-incubated with 10 μM NAD+ or ADP-ribose (ADPr) before application on membrane. B) AR immunoprecipitation was conducted on extracts from androgen (R1881)- or vehicle-treated PC3 and PC3-AR cells and analyzed as in A).