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. 2021 Jan 21;9(1):e001807. doi: 10.1136/jitc-2020-001807

Figure 1.

Figure 1

Most T cells in cSCC are CCR7−CD45RA− TEMs. (A) Representative immunofluorescence microscopy images of cSCC stained for DAPI, CD3 and CD45RO, top row—low magnification, bottom row—high magnification. Dashed lines indicate tumor island borders. (B) Representative immunofluorescence microscopy images of cSCC stained for DAPI, CD4, CD8 and CD45RO. (A and B), Scale bars=50 µm. (C) Representative FACS plots from blood, normal skin (NS) and cSCC from the same patient showing expression of CCR7 (X-axis) and CD45RA (Y-axis) in CD3+, CD4+ and CD8+ gated populations. A schematic diagram is shown on the right, defining the naive, TEMRA, TCM and TEM by CCR7 and CD45RA expression as depicted. (D–F) Graphs showing percentages of CD3+ (D), CD4+ (E) and CD8+ (F) T cells that are naive, TEMRA, TCM and TEM from blood, NS and cSCC (n=14 tumors). Horizontal bars=means, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. cSCC, cutaneous squamous cell carcinoma; TCM, T-cell central memory; TEM, T-cell effector memory; TEMRA, T-cell effector memory re-expressing CD45RA.