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. 2021 Jan 6;9(1):44. doi: 10.3390/biomedicines9010044

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Expression of ALDH1B1 in the HT29 isogenic cell line pair. (a) ALDH1B1 gene expression levels detected by real-time PCR in HT29/mock and HT29/ALDH1B1 cells. (b) Evaluation of GFP+ cells (green) in HT29/mock and HT29/ALDH1B1 by flow cytometry analysis. Nuclei were stained with propidium iodide (PI) (red). Fluorescence microscopy for detecting the GFP+ cells (green) in HT29/mock (c) and HT29/ALDH1B1 (d) cells (upper panel at 40× magnification and lower panel at 100× magnification). Nuclei were stained with DAPI (4′,6-diamino-2-phenylindole) (blue). Results are expressed as mean ± SD of three independent experiments. *** p < 0.001.