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. 2021 Jan 6;10(1):103. doi: 10.3390/foods10010103

Table 1.

Characteristics of the probes used for FISH microscopy.

Probe EUB338 PF2 Strc-493 Lis-637
Specificity 90% of bacteria All yeast Streptococcus spp. Lactococcus spp. Listeria spp, except L. grayi
Target Gen 16S rRNA 18S rRNA 16S rRNA 16S rRNA
Position 338–355 618–636 493–511 637–657
Sequence 5′- GCT GCC TCC CGT AGG AGT -3′ 5′- CTC TGG CTT CAC CCT ATT C -3′ 5′- GTT AGC CGT CCC TTT CTG G -3′ 5′- CAC TCC AGT CTT CCA GTT TCC-3′
Fluorophore Fluorescein Texas red Cy3 Fluorescein