Table 1.
Probe | EUB338 | PF2 | Strc-493 | Lis-637 |
---|---|---|---|---|
Specificity | 90% of bacteria | All yeast | Streptococcus spp. Lactococcus spp. | Listeria spp, except L. grayi |
Target Gen | 16S rRNA | 18S rRNA | 16S rRNA | 16S rRNA |
Position | 338–355 | 618–636 | 493–511 | 637–657 |
Sequence | 5′- GCT GCC TCC CGT AGG AGT -3′ | 5′- CTC TGG CTT CAC CCT ATT C -3′ | 5′- GTT AGC CGT CCC TTT CTG G -3′ | 5′- CAC TCC AGT CTT CCA GTT TCC-3′ |
Fluorophore | Fluorescein | Texas red | Cy3 | Fluorescein |