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. 2021 Jan 6;22(2):499. doi: 10.3390/ijms22020499

Figure 4.

Figure 4

Effects of anthocyanins on intracellular signaling pathways. Cells were incubated in the absence (control) and presence of 50 µM anthocyanins for 1 h. (A) Cell lysates were immunoblotted with anti-p-Akt (Thr308), anti-p-Akt (ser473), and anti-Akt antibodies. (B) Cell lysates were immunoblotted with anti-p-ERK1/2, anti-ERK1/2, anti-p-Stat3, and anti-Stat3 antibodies. (C) Cell lysates were immunoblotted with anti-p-PI3K p85 (Tyr458), and anti-p-PI3K p85, anti-p-PDK1, and anti-PDK1 antibodies. The protein levels were represented as a percentage relative to control. n = 4. * p < 0.05 and NS p > 0.05 compared with control.