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. 2021 Jan 6;22(2):499. doi: 10.3390/ijms22020499

Figure 8.

Figure 8

Effects of anisomycin and SB203580 on CLDN2 expression. (A) Cells were incubated in the absence (vehicle) and presence of 10 µM SB203580 (SB) for 24 h. Cell lysates were immunoblotted with anti-CLDN2 and anti-β-actin antibodies. The protein levels of CLDN2 are represented as a percentage relative to vehicle. (B) Cells were incubated with 10 µM CHX in the absence (control) and presence of 10 µM SB203580 (SB) for 0, 2, 4, and 6 h. The protein levels are represented relative to 0 h. (C) Cells were incubated in the absence (control) and presence of 10 µM C3G and 10 µM SB203580 for 6 h. The mRNA levels are represented as a percentage relative to control. n = 3–4. ** p < 0.01 and * p < 0.05 compared with 0 µM or control. NS p > 0.05 compared with vehicle.