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. 2021 Jan 6;26(2):255. doi: 10.3390/molecules26020255

Figure 5.

Figure 5

Effects of isookanin on activator protein 1 (AP-1) and nuclear factor κB (NF-κB) expression in LPS-induced RAW 264.7 cells. The (A) AP-1 and (B) NF-κB luciferase reporter vector was transfected into RAW 264.7 cells and cultured for 24 h. The cells were pretreated with isookanin for 2 h and then stimulated with LPS (200 ng/mL). Luciferase activity was calculated against an LPS-unstimulated control. The results are mean ± standard deviation (SD) (n = 3). # p < 0.01 vs. LPS-untreated control. * p < 0.05, and ** p < 0.01 vs. LPS-treated control.