Figure 4.

Cytotoxic and inhibitory effects of BG on ROS production in zebrafish embryos. Zebrafish eggs were incubated with BG in egg water for the indicated time (a). The survival rates of zebrafish eggs were determined by counting the hatched eggs (b). Zebrafish embryos were pretreated with the indicated concentration of BG for 24 h followed by additional 1 h H₂O₂ stimulation and the pictures were taken (c). Data are shown as the means ± SD of three independent experiments. Significant differences between different concentrations were analyzed using Duncan’s multiple range test with one-way ANOVA, and values labeled with different letters were significantly different (p < 0.05). White bar represents negative control. BG, black ginseng.