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. 2021 Jan 6;10(1):62. doi: 10.3390/antiox10010062

Table 2.

Mouse primer sequences used in reverse transcription PCR, real-time qPCR.

Gene Accession Number Primer Sequence (5′–3′) bp
RT-PCR BiP NM_001163434.1 Forward: CTG GGT ACA TTT GAT CTG ACT GG
Reverse: GCA TCC TGG TGG CTT TCC AGC CAT TC
398
CHOP NM_007837.4 Forward: CAC ATC CCA AAG CCC TCG CTC TC
Reverse: TCA TGC TTG GTG CAG GCT GAC CAT
286
IL-6 NM_001314054.1 Forward: CCG GAG AGG AGA CTT CAC AG
Reverse: GGA AAT TGG GGT AGG AAG GA
421
β-actin NM_007393.5 Forward: TCT CCA GCA ACG AGG AGA AT
Reverse: TGT GAT CTG AAA CCT GCT GC
348
Real-time
qPCR
TNF-α NM_013693.3 Forward: TGT CCC TTT CAC TCA CTG GC
Reverse: CAT CTT TTG GGG GAG TGC CT
IL-6 NM_001314054.1 Forward: GGG ACT GAT GCT GGT GAC AA
Reverse: TCC ACG ATT TCC CAG AGA ACA
β-actin NM_007393.5 Forward: GACGTTGACATCCGTAAAG
Reverse: CAGTAACAGTCCGCCT

RT-PCR, reverse transcription polymerase chain reaction; BiP, binding immunoglobulin protein; CHOP, CCAAT/enhancer-binding homologous protein; IL-6, interleukin-6; real-time qPCR, real-time quantitative polymerase chain reaction; TNF-α, tumor necrosis factor-α; NF-κB, nuclear factor kappa light chain enhancer of activated B cells.