Table 2.
Gene | Accession Number | Primer Sequence (5′–3′) | bp | |
---|---|---|---|---|
RT-PCR | BiP | NM_001163434.1 | Forward: CTG GGT ACA TTT GAT CTG ACT GG Reverse: GCA TCC TGG TGG CTT TCC AGC CAT TC |
398 |
CHOP | NM_007837.4 | Forward: CAC ATC CCA AAG CCC TCG CTC TC Reverse: TCA TGC TTG GTG CAG GCT GAC CAT |
286 | |
IL-6 | NM_001314054.1 | Forward: CCG GAG AGG AGA CTT CAC AG Reverse: GGA AAT TGG GGT AGG AAG GA |
421 | |
β-actin | NM_007393.5 | Forward: TCT CCA GCA ACG AGG AGA AT Reverse: TGT GAT CTG AAA CCT GCT GC |
348 | |
Real-time qPCR |
TNF-α | NM_013693.3 | Forward: TGT CCC TTT CAC TCA CTG GC Reverse: CAT CTT TTG GGG GAG TGC CT |
|
IL-6 | NM_001314054.1 | Forward: GGG ACT GAT GCT GGT GAC AA Reverse: TCC ACG ATT TCC CAG AGA ACA |
||
β-actin | NM_007393.5 | Forward: GACGTTGACATCCGTAAAG Reverse: CAGTAACAGTCCGCCT |
RT-PCR, reverse transcription polymerase chain reaction; BiP, binding immunoglobulin protein; CHOP, CCAAT/enhancer-binding homologous protein; IL-6, interleukin-6; real-time qPCR, real-time quantitative polymerase chain reaction; TNF-α, tumor necrosis factor-α; NF-κB, nuclear factor kappa light chain enhancer of activated B cells.