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. 2019 Oct 4;116(10):1742–1755. doi: 10.1093/cvr/cvz251

Figure 1.

Figure 1

Cardiac-specific Trx1 deletion causes early death. (A) Schematic representation of the strategy for knockout of Trx1. (B) Exon 2 contains the catalytic centre (Cys 32 and 35), indicated by yellow triangles. Other cysteine residues are indicated by grey triangles. (C) Trx1 mRNA lacking Exons 2 and 3 are expressed in Trx1cKO mice. (D) Schematic representation of the truncated Trx1 mRNA expressed in the cKO mice. (E) Mendelian ratios of Trx1cKO and global Trx1KO mice. Genotype of mating pairs and number of animals for the indicated mouse genotypes are shown. Genomic DNA for genotyping of pups was extracted at 1–3 weeks of age. (F) Full length Trx1 protein is down-regulated in the cKO mice. (G) High mortality of the Trx1cKO mice. Statistical analysis was performed with the Kaplan–Meier log rank test.