Figure 4.

Downregulation of genes involved in fatty acid metabolism and mitochondrial ATP production in Trx1cKO mice. (A) Expression levels of the indicated genes were examined with RNA-seq. The mean value from wild-type mice was expressed as 1 (N = 3). (B) Downregulation of indicated mitochondrial proteins, as shown by western blot analyses. (C) Electron microscopic analyses of Trx1cKO mice. Representative electron microscopic images are shown. Mitochondrial size, aspect ratio, and number were determined from the electron microscopic images (N = 5). Statistical significance was determined with the Student’s t test (A and C). *P<0.05, **P<0.01, and ***P<0.001.