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. 2021 Jan 23;10(3):e12053. doi: 10.1002/jev2.12053

FIGURE 1.

FIGURE 1

CFTR gene activation using Zinc Finger activator protein. (A) A schematic of the zinc finger binding domain targeted by CFZF in the CFTR promoter. The target sequence is shown along with its genomic location as determined from the UCSC genome browser. (B) Schematic of vector expressing the FLAG‐CFZF‐VPR. A CMV Pol II promoter expresses a FLAG‐tagged CFZF targeted to the CFTR promoter with a VPR activation domain. (C) ChIP assays were performed and enrichment relative to control (Neg) is shown as a fraction of input. (D) CFTR mRNA levels as determined by qRT‐PCR in HuBECs from healthy donor (HuBEC CFTR‐WT), and (E) HuBECs from CF patient with the F508del mutation (HuBEC CFTR‐F508del). HuBEC non‐transfected were used as negative control (Neg control). Experiments show the standard error of the mean and p values are represented (paired two‐sided Student's T‐test, *P = 0.01, **P < 0.02)