FIGURE 3.

Exosome mediated delivery of CFZF‐VPR increases CFTR protein expression and enhanced Chloride transport. CFTR ELISA analysis of CFTR protein expression in (A) HuBECs from healthy donor HuBEC–WT and (B) in patient cells with F508del mutation following transfection with CFZF‐VPR plasmid or treated with MSC‐derived exosomes carrying CFZF‐VPR. (C) A schematic is shown depicting the halide assay used to assess chloride transport. Fluorescence decrease was evaluated at 0, 2, and 60 s in response to exchange of 25 mM of sodium iodide in cells treated with 0.3 μM of forskolin and combination of VX‐809 and VX‐770, current drugs used for treatment of CF patients. (D) HuBEC CFTR‐WT and (E) HuBEC F508del cells were transfected with CFZF‐VPR plasmid or treated with MSC‐derived exosomes carrying CFZF‐VPR and assessed 48 h post‐treatment using the halide assay. Experiments were performed in triplicate with 10e+03 HuBEC treated with 5e+10 exosomes. ELISA and halide experiments were performed in triplicate in cells shown with the standard error of the means and p values from a paired two‐sided T‐test, *P < 0.05