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Optimizations of the full-length SARS-CoV-2 spike gene. (A) Distribution of codon usage frequency of the spike gene. Codon Adaptation Index (CAI) = 0.94. (B) Codon distribution percentage computed as codon quality group. (C) GC content adjustment with average equal to 55.69. (D) Restriction analysis for the S.opt.FL and S1.opt using single-cut digestion with BamHI and double-cut digestion with BamHI and NheI.
