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. 2021 Jan 6;14(1):39. doi: 10.3390/ph14010039

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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In vivo IFN-γ responses following C57BL/6 mice vaccinations. Comparing serum IFN-γ levels in each of vaccine constructs (S.opt.FL, S1.opt, and S.opt.FL+S1.opt) 2 weeks following second immunization in each vaccine construct using pooled mice sera from each group. Endpoint concentration was determined by titers expressed (mean ± SD). Data were analyzed with one-way ANOVA with Tukey’s multiple comparison test. The asterisks refer to the level of significance: *** p < 0.0002; ns: no significant difference.