Figure 2.

Silver nanoparticles (AgNPs) antiproliferative activity on human adenocarcinoma HCT-15. Cell viability (a) was determined using 5.5 µM, 55.6 µM, 556.2 µM, and 5.5 mM (0.6, 6, 60 and 600 μg/mL) of metallic silver contained in AgNPs. (b) Apoptosis, (c) necrosis, (d) reactive oxygen species (ROS) overproduction within the cell, and (e) ROS overproduction inside mitochondria were determined using half of the IC₅₀, IC₅₀, and double of the IC₅₀. The * represents a significant difference (p ≤ 0.001) compared with negative control, ** represents a significant difference (p ≤ 0.001) compared with carboplatin.