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. 2021 Jan 6;9(1):45. doi: 10.3390/biomedicines9010045

Figure 4.

Figure 4

PKCι is activated by PS, PA, and PI(4)P. (A) The activity of commercial purified GST-PKCζ was measured in the presence of 50-µg/mL PS or PA or PI(4)P. A complete reaction without an enzyme is considered as the negative control. (B) The activity of commercial purified GST-PKCι was measured in presence of 50-µg/mL PS or PA or PI(4)P. A complete reaction without an enzyme is considered as the negative control. (C) The activity of in-house purified GST-PKCι and deletion mutants was measured in presence of 0.5-ng/mL PS or PA or PI(4)P. Mock purification and no enzyme conditions are used as negative controls. Data are the mean ± SEM of at least 4 independent experiments performed in triplicate. A single, double, triple and four asterisks denote their significance of p-value ≤ 0.05, ≤ 0.01, ≤ 0.001 and ≤ 0.0001 respectively, ns mean No significant.