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. 2021 Jan 7;11(1):69. doi: 10.3390/biom11010069

Figure 2.

Figure 2

CETP reduces Caveolin/NOS association and increases eNOS phosphorylation. Representative images of mouse aortas (A) showing the effects of CETP on NOS/Caveolin interactions, and quantification is shown below. Results are Mean ± SEM, n = 4–5 * p <0.05 (scale bar = 50 µm). (B) Western-blot analysis of phosphorylated (Ser1117) and total eNOS in aortas from CETP transgenic and non-transgenic (Ntg) mice or (C) HAECs transfected with siCETP or control scrambled siRNA. (D) Phosphorylated and total AKT1 and ERK1/2 in HAECs. Results are mean ± SEM, n = 3. * p <0.05. (E) HAECs transfected with siCETP or control scrambled siRNA were stained with filipin or fluorescent-labeled CTB to detect levels of surface membrane lipids. Quantification of filipin or CTB binding efficiency (n = 10, 2 experiments, * p < 0.001), scale bar = 10 µm. HAECs, human aortic endothelial cells. CETP, cholesteryl ester transfer protein. NS, non-silence. siCETP, silenced CETP. CTB, cholera toxin subunit B.