Figure 4.

Design and subtypes of bispecific antibodies. BsAbs can be classified as BsAbs containing two Fab domain connected by a linker domain and those containing a Fc domain. BsAbs containing only Fab domains like BiTE (Panel (A)) are the smallest and simpler type and activate T-cells inducing TCR clustering. BsAbs containing a Fc region, instead, can exhibit antibody specific functions like ADCC and ADCP and can be further distinguished in those with IgG antibody structure and those containing additional binding sites that improve the target recognition (Panel (B,C)). BsAbs: bispecific antibodies. TCR: T cell receptor. MHC: major histocompatibility complex. ADCC: antibody dependent cellular cytoxocity. ADCP: antibody dependent cellular phagocytosis.