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. 2021 Jan 7;10(1):67. doi: 10.3390/antiox10010067

Figure 5.

Figure 5

The effect of Resvega (R10 μM; DMSO 1% v/v) on the caspase-1 activity in conjunction with MG-132 (MG; 5 μM) and bafilomycin A1 (BafA; 50 nM) exposure in IL-1α-primed ARPE-19 cells. The caspase-1 activity was investigated using a specific fluorochrome inhibitor of caspase-1 (FLICA, FAM-YVAD-FMK) and detected in a fluorescent microscope (Zeiss ApoTome.2 Imager M2 microscope). A green fluorescent signal (pointed as an example by a red arrow) indicates the presence of active caspase-1 attached to the FAM-YVAD-FMK-probe. Nuclei were stained using the blue Hoechst 33342 dye. Resvega was diluted by DMSO which served as the diluent control to Resvega, and IL-1α for IL-1α-primed cells with inflammasome activators MG-132 and BafA. One representative picture is presented from each group (a more extensive combination of pictures is included in the Supplementary Materials; Figures S1–S3).