Figure 6.

MTT and proliferation assay: (A) There was no difference between the viability of hASCs, which were labeled with 0.015 mM (light grey columns) and 1.5 mM VSOPs (dark grey columns), and unlabeled cells (white columns) immediately after the labeling procedure and after 24, 48 and 72 h. The values of the unlabeled cells were normalized to a viability of 100% for each single patient. Mean extinction values for each patient and concentration were normalized to the respective values of unlabeled hASCs from the same patient. Human ASCs treated with tert-butylhydroperoxide (t-BHP) (black columns) showed a significant decrease in cell viability compared to VSOPs-labeled and unlabeled hASCs. Significance is indicated by asterisks (* p < 0.0001). (B) VSOPs-labeled hASCs showed no difference in proliferation capacity compared to the unlabeled controls.