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. 2021 Jan 7;9(1):29. doi: 10.3390/vaccines9010029

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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IFNγ response assessed by ELISpot assay using fibroblasts transfected with the pCMV-Ub-MGF505-7R, pCMV-Ub-A238L and pCMV-Ub-MGF100-1L plasmids as APCs, and PBMCs from ASF-convalescent animals as effector cells (pig individual numbers on the x axis). Values represented correspond to the specific IFNγ-SC (spots) found after stimulation with autologous fibroblasts transfected with each specific plasmids, once subtracted the number of spots found with pCMV-Ub (empty plasmid) transfected fibroblasts (always <10). Levels of specific IFNγ-SC observed after stimulation with the BA71ΔCD2 virus are also shown.