Fig. 4.

Immunoprecipitation of the NS6 protein from PDCoV-infected cells or purified virions by anti-NS6-pt. Mock-infected cells (lane 1), PDCoV-infected cells (lane 2), or purified virions (lane 3) were subjected to immunoprecipitation analysis using (A) rabbit IgG (as the negative control), (B) anti-N (as the positive control), and (C) anti-NS6 antibody, respectively. The immunoprecipitated proteins were probed with anti-NS6-pt or anti-N antibody. (D) Whole cell lysates were analyzed by Western blot analysis using the anti-M antibody. IP: immunoprecipitation; WB: Western blot; WCL: Whole cell lysate; IgG (H): IgG heavy chain.