Table 1.
Summary of PDCoV antibodies used in the study.
| Name of Ab | Type | Antigen | Source animal | Applicationa |
|---|---|---|---|---|
| Anti-NS6 | pAb | Recombinant NS6 protein | Mouse | IFA, IHC, IE, VN |
| Anti-NS6-pt | pAb | Synthetic NS6 peptide | Rabbit | IFA, WB, IP |
| Hyperimmune serum | pAb | Purified PDCoV virions | Rabbit | WB, IEM, VN |
| Anti-S1 | pAb | Recombinant S1 protein | Rabbit | IEM |
| Anti-M | pAb | Synthetic M peptide | Rabbit | IHC, WB |
| Anti-Nb | mAb | Recombinant N protein | Mouse | IFA, IP |
a
IFA: Immunofluorescence assay; IHC: Immunohistochemistry; IEM: Immunoelectron microscopy; WB: Western blot; IP: Immunoprecipitation; VN: Virus neutralization.
b
Purchased from Medgene Labs, USA.