Fig. 7.

The activation of HIF-1α/HO-1 signaling pathway improved Golgi stress in vitro. The MLE12 cells were pretreated with 100 μM DMOG for 24 h and then incubated with LPS for another 24 h to increase the expression of HIF-1α and HO-1 in MLE12 cells. The results showed that DMOG treatment increased cell viability (Fig. 7A) and SOD activity (Fig. 7C) and decreased ROS expression (Fig. 7B) when compared with LPS group. The Western blot was used to measure the protein levels of HIF-1α, HO-1, GM130, mannosidaseⅡ, Golgin97 and GOLPH3 in vitro (Fig. 7D–J). *P < 0.05 vs. control, ^#P < 0.05 vs. LPS group. N = 3 at least for each group. Data were presented as mean ± SD, and were analyzed by one-way analysis of variance (ANOVA), followed by the Bonferroni correction post hoc test.*.